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NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT <t>markers</t> <t>(PAI‐1,</t> <t>Vimentin,</t> N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.
Primary Antibodies Against Total Smad2/3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against total smad2/3 - by Bioz Stars, 2026-10
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primary antibodies against total smad2, p-smad2 or e-cadherin  (Cell Signaling Technology Inc)
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NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT <t>markers</t> <t>(PAI‐1,</t> <t>Vimentin,</t> N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.
Primary Antibodies Against Total Smad2, P Smad2 Or E Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against total smad2 3  (Cell Signaling Technology Inc)
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NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT <t>markers</t> <t>(PAI‐1,</t> <t>Vimentin,</t> N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.
Primary Antibodies Against Total Smad2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against total smad2  (Thermo Fisher)
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NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT <t>markers</t> <t>(PAI‐1,</t> <t>Vimentin,</t> N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.
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primary antibodies against total smad2 - by Bioz Stars, 2026-10
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primary antibodies against total and phosphorylated smad2, smad3, smad4 and gapdh  (Cell Signaling Technology Inc)
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NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT <t>markers</t> <t>(PAI‐1,</t> <t>Vimentin,</t> N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.
Primary Antibodies Against Total And Phosphorylated Smad2, Smad3, Smad4 And Gapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against total and phosphorylated smad2, smad3, smad4 and gapdh - by Bioz Stars, 2026-10
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NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT markers (PAI‐1, Vimentin, N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Molecular Mechanisms of Neutrophil Extracellular Traps in Promoting Gastric Cancer Epithelial–Mesenchymal Transition Through SERPINE‐1 Expression

doi: 10.1002/jbt.70157

Figure Lengend Snippet: NETs promote EMT in gastric cancer cells. (A) Transwell invasion assay and wound healing assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images at 48 h were shown. (B) Western blot analysis of EMT markers (PAI‐1, Vimentin, N‐cadherin, E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in HGC‐27 and MKN‐45 cells treated with NETs. Scale bar: 20 μm. (C) Sphere formation assay and CCK‐8 proliferation assay of HGC‐27 and MKN‐45 cells treated with NETs or DNase‐1‐treated NETs. Representative images of sphere formation assay at 48 h were shown.

Article Snippet: The membranes were blocked with 5% non‐fat milk and then incubated with primary antibodies against PAI‐1, Vimentin, N‐cadherin, E‐cadherin, TGF‐β1, TGF‐βR1, TGF‐βR2, phospho‐Smad2/3, Smad4 and total Smad2/3 (all 1:1,000, Cell Signaling Technology) overnight at 4°C.

Techniques: Transwell Invasion Assay, Wound Healing Assay, Western Blot, Immunofluorescence, Staining, Tube Formation Assay, CCK-8 Assay, Proliferation Assay

SERPINE‐1 knockdown reverses NET‐induced EMT in gastric cancer cells. (A) Western blot analysis confirming SERPINE‐1 knockdown efficiency in HGC‐27 and MKN‐45 cells. (B) Western blot analysis of EMT markers in control and SERPINE‐1 knockdown cells treated with NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in control and SERPINE‐1 knockdown cells treated with NETs. Scale bar: 20 μm. (C) Transwell invasion assay and wound healing assay of control and SERPINE‐1 knockdown cells treated with NETs. Representative images at 48 h were shown. (D) CCK‐8 proliferation assay of control and SERPINE‐1 knockdown cells treated with NETs.

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Molecular Mechanisms of Neutrophil Extracellular Traps in Promoting Gastric Cancer Epithelial–Mesenchymal Transition Through SERPINE‐1 Expression

doi: 10.1002/jbt.70157

Figure Lengend Snippet: SERPINE‐1 knockdown reverses NET‐induced EMT in gastric cancer cells. (A) Western blot analysis confirming SERPINE‐1 knockdown efficiency in HGC‐27 and MKN‐45 cells. (B) Western blot analysis of EMT markers in control and SERPINE‐1 knockdown cells treated with NETs. Additionally, immunofluorescence co‐staining of PAI‐1 and EMT typical markers (Vimentin, N‐cadherin, and E‐cadherin) in control and SERPINE‐1 knockdown cells treated with NETs. Scale bar: 20 μm. (C) Transwell invasion assay and wound healing assay of control and SERPINE‐1 knockdown cells treated with NETs. Representative images at 48 h were shown. (D) CCK‐8 proliferation assay of control and SERPINE‐1 knockdown cells treated with NETs.

Article Snippet: The membranes were blocked with 5% non‐fat milk and then incubated with primary antibodies against PAI‐1, Vimentin, N‐cadherin, E‐cadherin, TGF‐β1, TGF‐βR1, TGF‐βR2, phospho‐Smad2/3, Smad4 and total Smad2/3 (all 1:1,000, Cell Signaling Technology) overnight at 4°C.

Techniques: Knockdown, Western Blot, Control, Immunofluorescence, Staining, Transwell Invasion Assay, Wound Healing Assay, CCK-8 Assay, Proliferation Assay